Attachment of Idarubicin to Glutaraldehyde-coated Magnetic Nanoparticle and Investigation of its Effect in HL-60 Cell Line

Idarubicin is a chemotherapeutic drug frequently used to treat breast cancer and acute leukemia. This study aimed immobilize idarubicin on glutaraldehyde (GA)-coated magnetic nanoparticles (MNP-GA) prepare with high stability low toxicity. We prefreed MNPS because of their easy synthesis, cost, non-toxicity. In the study, magnetite (Fe3O4) were prepared, coated glutaraldehyde, characterization processes performed Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction pattern (XRD), Conventional transmission electron microscopy (C-TEM) methods, (IDA) was bound. The cytotoxic effects idarubicin-bound MNP-GA free HL-60 cell lines determined by MTT ATP tests, IC50 values calculated. Flow cytometry evaluate apoptosis status, expression MDR1, Puma, NOXA, BAX, Survivin, BCL-2 genes measured polymerase chain reaction (PCR). It found that decreased between 5 7 times use MNP. PCR expressions apoptotic increased, while MDR1 anti-apoptotic in Apoptosis be increased flow measurements. MNP systems has reduced resistance since it provides controlled release prevents its exit from due structure.